Tranquill first appeared in A Rival Battle for Club Champ! where it was the first Pokemon Trip used in his second battle with Ash in the Battle Club. It easily defeated Ash's Tepig and Oshawott with its Super Luck-boosted Aerial Ace before losing to Pikachu.
It appeared again in A Venipede Stampede! where it saved Ash's Pidove by using Air Cutter to block a Venipede's Sludge Bomb in Castelia City. It then teamed up with Ash's newly-evolved Tranquill, using Aerial Ace to send the other Venipede back to their colony.