Serperior first appeared, as a Snivy, in BW001: The Shadow of Zekrom!, as one of Professor Juniper's starters to be distributed to the beginner trainers in Unova. Now belonging to Trip, Snivy battled Ash's Pikachu and won due to Pikachu being unable to use any electric attacks.
He appeared again in BW010:A Rival Battle for Club Champ! during Trip's second battle with Ash, where it was revealed to have evolved into a Servine. He battled Pikachu and easily defeated him with a Cut attack, before losing to Ash's Snivy after being hit by Attract.
In BW092, it is revealed that Servine has evolved into Serperior and that he will compete in the World Tournament Junior Cup and his first opponent was Burgundy and her Darmanitan. Serperior was quickly able to knock out Darmanitan with a Leer and Solar Beam combination in only one turn.
In BW093, it was seen having a battle with Manning's Heatmor and Cilan's Crustle. In its battle with Crustle, it wins with a combination of Leer-Solarbeam-Frenzy Plant to be the victor.
In BW094, it is seen battling Ash's Pignite. Pignite attacks with Tackle and its two Fire type moves: Flamethrower and Flame Charge. Serperior lands a powerful Solarbeam before Pignite's Fire Pledge. Serperior comes in and finishes it with Frenzy Plant. It was Sionara, Pignite after being hit by 2 thorns.
It also has a battle against Alder's Bouffalant. After Leer, Solarbeam, and Dragon Tail, Alder's Bouffalant comes in with a finisher Head Charge.
In BW105-BW106, it battled its rival, Ash's Pikachu. After dodging Quick Attack, Iron Tail, and Electro Ball, Serperior strikes with a mirage of Dragon Tail, as well as using Energy Ball and Wrap. Serperior lost after a collision between Dragon Tail and Iron Tail/Electro Ball.
|Tackle||In the Shadow of Zekrom!|
|Leaf Tornado||In the Shadow of Zekrom!|
|Cut||A Rival Battle for Club Champ!|
|Leer||Jostling for the Junior Cup!|
|SolarBeam||Jostling for the Junior Cup!|
|Frenzy Plant||Battling Authority Once Again!|
|Dragon Tail +||Ash, Iris and Trip: Then There Were Three!|
|Energy Ball +||BW106|
|+ indicates this Pokémon used this move recently.*|
- indicates this Pokémon normally can't use this move.
- Japanese: Shin-ichiro Miki (as Snivy), Kiyotaka Furushima (as Servine).
- English: Jason Griffith.