Venusaur first appeared in an image with its trainer when Sanpei was talking about his brothers. Nihei later used Venusaur when Nihei challenged Sanpei for a battle. Sanpei's Greninja started the battle by using Double Team and Venusaur countered the attack by using Vine Whip. Venusaur used Razor Leaf and Greninja countered the attack with Water Shuriken. Both attacks collided and Greninja appeared in front of Venusaur, using Night Slash and hitting Venusaur. Greninja used Night Slash once more but Venusaur disappeared by using Petal Blizzard. Venusaur appeared above Greninja and used Razor Leaf, hitting Greninja. Nihei then ordered Venusaur to use Solar Beam and that attack knocked out Greninja. Hanzo later revealed to Sanpei that Venusaur used Vine Whip to weaken the attack of Greninja's Night Slash which surprised Sanpei. Venusaur was later used again when the Ninja Village was under attack by Kagetomo's army, protecting the village and the villagers.