Still on their way to Olivine City Ash and co. decide to take another break. They notice Brock's Golbat acting weird and follow it. They notice that the reason for Golbat acting weird was because of a sonic wave emitting machine belonging to a young woman named Tierra, who was looking for two artifacts belonging to an evil queen who had the power to control Pokémon.
However, Team Rocket finds out about this and goes underground. When they get inside Jessie puts on the mask and becomes the new Queen of Pokémon. Can Ash and co. stop her? And why are there tiles lined up neatly on a section of ground?