Cacturne made its first appearance in A Cacturne for the Worse, along with Harley. Harley and Cacturne participated in the Izabe Island Pokémon Contest, making it all the way to the final, before being beaten by May and her Bulbasaur. After the Izabe Island Contest, Harley used Cacturne once again during the Hoenn Grand Festival against May. Along with his Banette, he battled against her in a double battle, but he lost once again.
Harley also used Cacturne in the Appeal Stage of the Saffron City Contest in A Hurdle for Squirtle. Harley lent Cacturne to Jessie to use in the appeal round of the Mulberry City contest during New Plot, Odd Lot. After Harley sent it out, it automatically fell in love with Jessie. Harley explained this happened because he (Harley) and Jessie were soul-mates.
Harley used Cacturne in the Kanto Grand Festival in a battle against May where it battled along with Harley's Wigglytuff. It took on her Munchlax and Eevee but was quickly defeated with a combination of Focus Punch and Shadow Ball, respectively.The last time Cacturne was seen was during The Unbeatable Lightness of Seeing where it was used to expose Team Rocket and send them blasting off after May and Drew's battle.