Gardenia's Turtwig first appeared while Ash and his friends were heading towards Eterna City. When Gardenia challenged Ash, she used her Turtwig who displayed awesome speed, being about twice as fast as Ash's Turtwig, which was also quite agile for its species. Gardenia's Turtwig defeated Ash's Turtwig and Staravia without taking any damage, even managing to avoid Staravia's Aerial Ace. Turtwig was later used to help protect the Adamant Orb.
During Gardenia's Gym battle with Ash, Turtwig once again displayed incredible speed, and managed to defeat Staravia a second time. She then battled Ash's Turtwig again but this time she lost.
Later, when Gardenia and James challenged Ash and Dawn to a double battle, Turtwig fought alongside James' Cacnea. However Gardenia lost focus during the battle, resulting in Turtwig's defeat at the hands of Dawn's Pachirisu.
|Leaf Storm||The Grass Type Is Always Greener|
|Tackle||The Grass Type Is Always Greener|
|Leech Seed||The Grass Type Is Always Greener|
|Bite||The Grass Type Is Always Greener|
|+ indicates this Pokémon used this move recently.*|
- indicates this Pokémon normally can't use this move.