Gardenia's Turtwig first appeared while Ash and his friends were heading towards Eterna City. When Gardenia challenged Ash, she used Turtwig who displayed awesome speed, being about twice as fast as Ash's Turtwig, which was also quite agile for a Turtwig. Gardenia's Turtwig defeated Ash's Turtwig and Staravia without taking any damage, even managing to avoid Staravia's Aerial Ace. Turtwig was later used to help protect the Adamant Orb.
During Gardenia's Gym battle with Ash, Turtwig once again displayed incredible speed, and managed to defeat Staravia a second time. Turtwig then battled Ash's Turtwig again but this time, was defeated.
Later, when Gardenia and James challenged Ash and Dawn to a double battle, Turtwig fought alongside James' Cacnea. However Gardenia lost focus during the battle, resulting in Turtwig being defeated by Dawn's Pachirisu.