Fergus introduced his Pokémon, including Nidoqueen, to Ash Ketchum and his friends. Nidoqueen was later captured by Mewtwo by using the Mewtwo Ball. Nidoqueen was later freed along with the rest of the Pokémon by Ash. Nidoqueen then battled against the Nidoqueen clone.
None of Nidoqueen's moves are known.