Mightyena debuted in Oaknapped!, where it went up against Tracey's Scyther and Ritchie's Taillow alongside Cassidy's Sableye. It appeared again in A Date With Delcatty, where it battled Casey's Meganium. Its last appearance was in Luvdisc Is a Many Splendored Thing!, where it battled Luverin and ended up accidentally blasting off Butch and Cassidy with a Hyper Beam. It was Butch's first known Hoenn Pokémon.