Since the Gym in Opelucid City was closed, Ash had to use the Virbank City Gym as a substitute and here he meets the ultra-punky Roxie who has a Koffing (from Ash's home region in Kanto while they perform a rock concert). And introducing her band, "Koffing and the Toxics." He find out soon enough that the Virbank Gym doubles as a live venue. With a loud Thunderbolt from Pikachu to rock their socks off! Ash challenges the young Poison-type Gym Leader to a Poisonous Gym Battle in which she accepts with one condition: Ash will battle with a full team of six Pokémon while she uses only three Poison-type Pokemon. What Poison-type Pokémon strategy will this next generation Pretty Poison have up her sleeve? The Virbank Gym 6 on 3 battle begins now!
Prior to this episode, Ash swapped Oshawott, Snivy, Scraggy and Krokorok for Boldore, Unfezant, Leavanny and Palpitoad.
Although Krokorok got swapped with Boldore first from the last episode.
Roxie is the first Gym Leader who made her anime debut prior to the game she was in. (Pokémon Black 2 and Pokémon White 2)